New Cultivation Technology Explores the Phycosphere

A schematic diagram of the porous microplate between culture wells. This allows microalgae and bacteria to be cultured in wells at different distances from one another and exchange nutrients without being in physical contact. Image courtesy of Hyungseok Kim, Massachusetts Institute of Technology

The water immediately outside microalgal cells, called the phycosphere, is rich with organic carbon that is secreted by the algae. The area is an ideal ecosystem for the growth of bacteria. However, detecting and measuring bacterial cell activity and population growth in the phycosphere is difficult. This is because until now scientists have not been able to track single bacteria, and their locations in relation to algal cells, over the course of an algal population growth cycle.

In Department of Energy sponsored research, scientists are now exploring new ways to study how the phycosphere structures bacterial communities across time and space. To do this, the researchers have created a new co-culture method called a “porous microplate.”

Porous Microplate Technology

In the lab, microplates sit in the walls between culture wells, which are tiny pools where microalgae and bacteria grow. The microplate is made of a material with nanoscale pores, smaller than the microalgae and bacteria. The tiny pores mean the microplates can pass nutrients and molecules associated with metabolism between culture cells while blocking physical contact between algae in adjacent wells.

This design enabled the researchers to stretch out the phycosphere in both space and time. As a result, they could quantify how bacteria grow and how the bacterial community changes at different distances from the algal cells.

After sequencing the DNA of algal microbiome cultured in the microplate, the team found that certain bacteria responded to the algal production of organic carbon in a spatially dependent manner. Specifically, they found that bacteria associated with the algae reached higher abundances when placed closer to the algal culture well. This result fits with expectations for real phycosphere environments.

The researchers also unexpectedly found that cultivation of the diatom Phaeodactylum in the microplate led to yields 20 times greater than batch cultures, due to continuous supplementation of nutrients.

The new incubation method is highly effective for algal cultivation, allowing the diatom Phaeodactylum to accumulate to its theoretical physical limit, densely packed with cell-to-cell distances equal to their cell radii. This result may be important to efforts to produce increased and more efficient algal biomass production at large scales.

Moreover, the porous microplate system facilitates investigation of community-level microbial interactions in complex small-scale ecosystems mediated by metabolite exchange. The system shows that the algal phycosphere is a complex ecosystem which allows multiple microbial groups to thrive in different locations within this micro-scale environment.

Ref: Bacterial response to spatial gradients of algal-derived nutrients in a porous microplate.
Kim, Hyungseok, Kimbrel, Jeffrey A., Vaiana, Christopher A., Wollard, Jessica R., Mayali, Xavier, and Buie, Cullen R.
United Kingdom: N. p., 2021. Web. doi:10.1038/s41396-021-01147-x.

All rights reserved. Permission required to reprint articles in their entirety. Must include copyright statement and live hyperlinks. Contact Algae Planet accepts unsolicited manuscripts for consideration, and takes no responsibility for the validity of claims made in submitted editorial.

seagricultureusa 2023 Portland ME



  • March 28, 2023: South Korean biotechnology startup Seawith recently signed a new partnership to assess and explore Seawith’s proprietary, cutting-edge microalgae technologies in commercially cultivated meat projects. Read More…
  • March 23, 2023: Provectus Algae has announced that Steven F. Schnittger has joined the company as a cosmetics industry advisor. Mr. Schnittger is the former Vice-President of Global Fermentation and Microbiology R&D at The Estée Lauder Companies Inc. and served over 37 years there. Read More…
  • March 21, 2023: Testa Omega-3 has launched its Vrill Omega™ supplement, an innovative plant-based and vegan alternative to krill oil. It represents a new class of highly bioavailable omega-3 supplements offering both essential polyunsaturated fatty acids (DHA and EPA), as well as the potent antioxidant astaxanthin, in a formulation that is rich in easily-absorbed polar lipids – phospholipids and glycolipids — derived from microalgae and plants. Read More…
Seaweed Industry Job Board

A Beginner’s Guide