
Medakamo hakoo growing on an agar plate. Green lines with dots show a pure culture of Medakamo hakoo streaked onto an agar medium. Study co-author Emeritus Professor Tsuneyoshi Kuroiwa first discovered the algae when the water in his home goldfish tank turned green after he added medaka fish (Japanese rice fish), and he decided to look at samples under his home microscope. However, where the algae originated from — the water, fish or somewhere else — is still unknown. ©2023 Sachihiro Matsunaga
Anew species of microalgae was found in water from a home aquarium. While analyzing DNA samples taken from the algae, researchers from the University of Tokyo discovered and named Medakamo hakoo, whose DNA sequence didn’t match any on record.
This new species is the smallest known freshwater green algae, with inherent qualities which enable it to be cultured stably at high density, meaning it could be effectively used to produce useful products for food and industry.
“We were very surprised to discover a new species of microalgae in just a regular home aquarium,” said Professor Sachihiro Matsunaga from the Graduate School of Frontier Sciences. “Alga were taken from the water and cultured one by one. The DNA of the alga was fluorescently stained and microscopically observed to find the one with the least amount of DNA per cell. We then sequenced the DNA of that alga and compared it to the DNA of other algae. The results did not match the DNA of any previously reported algae, indicating that it was a new species, and we named it Medakamo hakoo (M. hakoo).”

The smallest green algae in freshwater. This microscopic fluorescent image of a Medakamo hakoo shows a chloroplast (red), a nucleus (green) and a cytoplasm (blue) in the alga’s cell. The white scale bar indicates 500 nanometers (0.0005 millimeters). ©2023 Tsuneyoshi Kuroiwa
Microalgae are made up of relatively few genes, and this uncomplicated form makes them useful for researchers trying to identify what roles different genes play and how they could be used. Of the tens of thousands of known microalgae, many remain uncharacterized. Thanks to this latest study, we now know that not only is this a new species, but it also has the smallest known genome of any freshwater algae, as well as other useful qualities.
“M. hakoo contains only one mitochondrion (for producing energy) and one chloroplast (which contains chlorophyll and creates food through photosynthesis), whereas normal plant cells contain multiple mitochondria and chloroplasts. This indicates that it is a green alga with an extremely simple cell structure,” explained Dr. Matsunaga.
“From our research, we have also speculated that it has an unprecedented DNA structure and a new gene regulatory system,” he said. “Its cell cycle is also strongly synchronized with the day and night cycle, which is key to effective, stable bioproduction. Due to these inherent qualities and extremely small size, M. hakoo can be effectively cultured at high cell density, making it possible to mass produce substances such as highly functional foods, cosmetics, and biofuel at a low cost.”
The researchers plan to continue exploring the potential applications for M. hakoo, both in the lab and the wider world. “Aquatic green algae are the originating organisms of today’s land plants. Thanks to this research, we can better understand the minimum number of genes required for an organism to evolve and thrive in diverse environments, which we will continue to study,” said Dr. Matsunaga. “In the future, I would like to find ways to collaborate and create useful substances from the mass cultivation of M. hakoo.”
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